Custom Antibody Services
Eviogen Discovery delivers comprehensive custom antibody development and associated services, employing advanced immunological techniques and cutting-edge facilities. We are committed to providing high-quality antibody reagents for research and therapeutic applications with precision and reliability.
Polyclonal Antibody Production
Host Species: Rabbit, Chicken, Mouse
We specialize in generating high-titer, high-affinity polyclonal antibodies tailored to your specific requirements. Our approach guarantees robust immune responses and consistent antibody performance.
Key Features:
- Immunogen Requirements: Minimum 5 mg of antigen at ≥0.5 mg/mL concentration
- Immunization Protocol: Multi-site injections using optimized adjuvant regimens
- Screening: ELISA and Western blot validation
- Deliverables: Pre-immune & post-immune serum, optional purification
Monoclonal Antibody Development (Hybridoma Technology)
Host Species: Mouse
Our hybridoma-based monoclonal antibody production ensures highly specific antibodies against a broad spectrum of antigens. All animal procedures adhere strictly to CPCSEA ethical and regulatory standards.
Development Phases:
- Phase I - Immunization & Titer Evaluation (6-9 weeks): Immunization & ELISA titer check.
- Phase II - Hybridoma Generation & Screening (8-10 weeks): Fusion, HAT selection, ELISA screening.
- Phase III - Subcloning & Characterization (3-5 weeks): Monoclonality, expansion, isotyping.
- Phase IV - Production & Cryopreservation (3-4 weeks): Scale-up production & long-term storage.
Additional Services:
- Extra subcloning, expansion, and screening
- Cryopreservation of up to 20 clones per month
- Scaled antibody production (50-100 mL)
- Isotyping services
Antibody Purification
We offer tailored purification solutions for monoclonal and polyclonal antibodies using high-efficiency chromatography techniques.
Purification Options:
- Protein A/G affinity chromatography
- Protein L affinity chromatography
- Antigen affinity chromatography (requires antigen)
Sample Types: Serum, ascites, culture supernatant
Deliverables: Purified antibody with detailed purification report
Antibody Labeling Services
Enhance your antibody applications with our conjugation services.
Labeling Options:
- Enzymatic labels: HRP, ALP
- Fluorescent labels: FITC
- Biotinylation
All labeled antibodies undergo efficiency and functional integrity validation.
Single B-Cell Cloning & Lead Antibody Identification
Leveraging advanced sorting and molecular cloning technologies, we rapidly isolate and characterize monoclonal antibodies from individual B cells.
Highlights:
- Direct cloning & expression of paired Ig chains
- Lead candidates identified within 4 weeks
- Ideal for neutralizing antibody discovery
Service Summary
| Service | Host/Platform | Sample Requirement | Key Deliverables | Timeline |
|---|---|---|---|---|
| Polyclonal Antibody | Rabbit, Chicken, Mouse | ≥5 mg antigen (≥0.5 mg/mL) | Pre- & post-immune serum; optional purification | Custom (enquire) |
| Monoclonal Antibody (Hybridoma) | Mouse | 5 mg protein or 25-30 mg peptide | Culture supernatant, isotyping, clones, report | 20-28 weeks |
| Antibody Purification | N/A | Serum, supernatant, antigen (optional) | Purified antibody, QC report | 1-2 weeks |
| Antibody Labeling | N/A | Purified antibody | Labeled antibody, QC report | 1-2 weeks |
| Single B-Cell Cloning | Mouse, Human | PBMCs or splenocytes | Lead candidates, sequence data | 4 weeks |